Journal: ACS Chemical Neuroscience
Article Title: Fluorescent Filter-Trap Assay for Amyloid Fibril Formation Kinetics in Complex Solutions
doi: 10.1021/acschemneuro.5b00104
Figure Lengend Snippet: Application of the method. Top panels: effect of HSA on fibrillation kinetics of 4 μM A488-FAβ42 (ratio 1:500). (a) ThT traces of Aβ42 incubated with the indicated concentrations of HSA. (b) Filter-trap assay results for 0 μM (black), 3 μM (violet), 5 μM (pink), and 7.4 μM (navy) HSA of A488-FAβ42. The dashed lines are a guide to the eye. (c) Negative-staining EM image of A488-FAβ42 before the application of the filter-trap assay in the presence of 3 μM HSA. Bottom panels: Effect of 49 nm nominal size carboxyl modified polystyrene particles on the fibrillation kinetics of 3 μM IR680-FAβ42 (ratio 1:132). (d) ThT traces of Aβ42 incubated with the indicated concentrations of carboxyl modified polystyrene nanoparticles. (e) Filter-trap assay results for 0 mg/mL (black), 0.3 mg/mL (orange), and 0.6 mg/mL (violet) carboxyl modified polystyrene particles of IR680-FAβ42. The dashed lines are a guide to the eye. (f) Negative-staining EM image of IR680-FAβ42 before the filter-trap assay in the presence of 0.3 mg/mL carboxyl modified polystyrene particles. The scale bars represent 200 nm.
Article Snippet: Polystyrene nanoparticles of 49 nm nominal size with carboxyl surface modification were purchased from Polysciences, Inc.
Techniques: Incubation, TRAP Assay, Negative Staining, Modification